earticle

영어

LIM kinase(LIMK)s which includes LIMK1 and LIMK2, belongs to serine kinases and is discovered more than a decade ago, and LIMK is shown to be a regulator of actin dynamics. LIMKi3 is a specific inhibitor of LIMK including LIMK1 and LIMK2. Previous results show that LIMKi3 suppresses porcine oocyte maturation in vitro by affecting of actin distribution. However, the roles of LIMKi3 in porcine in early embryo stage embryos is unknown. In this study, we investigated the role of LIMK and LIMK inhibitor function in actin filament organization by affecting p-cofilin, And, we also investigated the cell junction assembly during early embryo development. Firstly, we confirmed LIMK and p-cofilin localization by immunocytochemistry and confocal microscopy. LIMK localized in cytoplasm and cell junction with F-actin after morula. p-Cofilin localized in cytoplasm during early embryo development. LIMKi3(10 uM) treatment with LIMKi3 after parthenogenetic activation, blastocyst rates was significantly decreased compared to the control group. After LIMKi3 treatment in morula, intensity levels of actin and p-cofilin, a downstream molecule of LIMK, were decreased in morula. Furthermore, cell junction related proteins were significantly decreased in LIMKi treatment. Thus, our result indicate that Limk interferes with porcine early embryo development and reduces actin formation by affecting cell junction assembly.