earticle

영어

In mammalian oocyte, acentriolar MTOC(Microtubule organizing center) surrounded by pericentriolar material (PCM) serve as the core structure of the centrosome. Acentriolar MTOC acts like a functional centriole in mouse oocyte. However, the underlying mechanisms remain poorly understood in mammalian germ cell. Here we show that Cep192, an evolutionarily conserved protein, is required for generation of a functional MTOC organizing spindle assembly in porcine oocyte. We found that Cep192 is recruited to the proximal acentriolar MTOC in metaphase I stage during porcine in vitro maturation. In particularly, Cep192 is expressed well in proximal regions of spindle at Anaphase I. Using injection of dsRNA against Cep192, Cep192 mRNA and proteins was decreased and depletion of Cep192 caused porcine oocyte maturation and impair spindle assembly in porcine oocyte. We also find that two cell cycle related kinase, polo-like kinase 1 (PLK1) and aurora kinase A (AURKA) are involved in the porcine oocyte maturation, indicating the common roles of cell cycle kinases in mammalian oocytes. Taken together, Cep192 is one of essential factor for the acentriolar spindle formations in porcine oocyte and indicating the conservation of roles of Cep192 in spindle assembly in mammalian meiotic cell division.