earticle

영어

Sperm treatment methods affect sperm fertility and developmental potential of in vitro fertilized(IVF) embryos. Confirmation of the developmental rate according to the information of donor cow will be helpful for the production of elite Jeju Black Cattle(JBC) embryos. To optimize the suitable JBC sperm treatment method, the following methods of percoll gradient, triladyl and percoll-triladyl combination(P, T and PT, respectively) were used to sperm treatment. At 2 days after IVF, there was no difference in the fertilization rate among all groups. The cleavage rate at day 4 was higher in PT and T groups than in P group. The blastocyst formation rate at day 8 was significantly increased in T and PT groups(p<0.05). In comparison of the in vitro development potential of donor and non-donor cows, there were no differences from IVF rate to blastocyst formation rate. According to JBC grade(1++, 1+, 1, 2), the development rate was the highest in 1++ grade cattle, followed by 1, 1+ and 2 grade. Also, in vitro developmental potential by carcass weight was the highest at the 300~350 kg, followed by 300 kg or less, 350~400 kg, and then 400 kg more over. While the T method can collect a small amount of viable sperm, the P method was present a little toxic to JBC sperm. Thus, we developed PT method combined two sperm treatment methods. In this study, the development of IVF embryos was more effective when the carcass grade was high and the carcass weight was about 350 kg. This result demonstrates that JBC sperm can be effectively recovered with simple and efficient PT method and using this technique, elite JBC IVF embryos can be produced in vitro for the mass production of JBC.