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영어

There were differences in reproductive physiology between miniature pig and normal pig, suggesting differences in estrus and pregnancy. The importance of apoptosis in the development of ovarian follicles and maturation of oocytes. However little is known about the molecular mechanism behind these characteristics. It is known that proper execution of programmed cell death is crucial for normal follicular development. Especially, the plays a very important role of remodeling corpus luteum for successful fertilization to reproductive effect of mammals. In the present study of this experiment was to understand the programmed cell death in normal pig and miniature pig corpus luteum of estrus day 15. Expression of MAP1LC3A and other autophagy- related genes (ATG13, Beclin-1) was significantly higher in normal pig corpus luteum. On the other hand, the expression of apoptosis-related genes (casp-3, BAX) and BCL-2 gene was significantly higher in miniature pig corpus luteum. Expression of genes related to cell metabolism activity (IGF, PCNA) was significantly higher in the normal pig corpus luteum than in the miniature pig corpus luteum. Gene expression of hormone receptor (FSH, LH) was higher than that of miniature pig corpus luteum. Our result is suggest that the expression of LH and FSH hormone in the normal pig is elevated by the high activity of the cell-metabolizing activity gene, and thus, the autophagy is induced. In the miniature pig, the expression of the cell metabolizing activity gene is low and the progesterone hormone mechanism And BCL-2 gene expression are thought to induce apoptosis. This is normal pig and miniature pig corpus luteum of estrus day 15 were able to confirm that shows a significantly different pattern to each other. While the process of programmed cell death in normal pig corpus luteum majorly depended on the type II pathway (autophagy), miniature pig corpus luteum preferred the type I pathway (apoptosis). These differences may contribute greatly to manipulating the reproduction of pigs.