earticle

영어

Matrix metallopeptidases (MMP) contribute to spatial and temporal matrix remodeling in the bovine endometrium. To test the this study that MMP expression in bovine Endometrial Epithelial Cells (bEECs) is regulated by implantation and embryo development in in vivo. Uterine bEECs impacts in structure and function in preparation for blastocyst implantation. We investigated the analysis of pre-implantation embryo development and quality on day 7 of gestation on the mRNA expression of MMPs and TIMPs in bEECs. bEECs and embryos were recovered 7 days after the first insemination by flushing the uterus with bEECs and Embryo Collection Medium. Next, we divided the bEECs sample into four groups: good (n=4), poor (n=5), low (n=3) and bad (n=2) samples and compared the group differences in MMP-2, and TIMP-2, TIMP-3 and their correlations within each group. The good group of samples: a total of 111 embryos were collected at the zona (n=5), non-fertilization (n=13), 2-cell (n=1), morula (n=7), compact morula (74) and early blastocyst (n=11). The poor group of samples: a total of 84 embryos were collected at the zona (n=3), non-fertilization (n=47), 4-cell (n=2), 8-cell (n=6), 16-cell (n=3), morula (n=2), compact morula (20) and early blastocyst (n=1). This low group of samples: a total of 10 embryos were collected at the zona (n=1), non-fertilization (n=1), 4-cell (n=2), 8-cell (n=1), 16-cell (n=2) and compact morula (n=3). The embryos collected not occur at bad group samples. To showed the average number of bEECs for the four groups, 5.6×10⁴ in the good group, 4.2×10⁴ in the poor group, 1×105 in the low group, and 8.0×10⁴ in the bad group of bEECs. The research was to examine MMP-2, TIMP-2, and TIMP-3 expression in the bovine EECs throughout gestation. The results imply that TIMP-2 is involved in very early local maternal recognition of pregnancy. In collected samples, the number of bEECs different behaviors of MMP-2, TIMP-2 and TIMP-3. Interestingly, active MMP2 in the endometrium may determine the allocation of growth factors supporting conceptus development. The presence of a day 7 conceptus in vivo and good samples mostly high expression than in another group samples endometrial TIMP2 mRNA expression. MMP are likely to participate in remodeling processes preparing a receptive endometrium for a timely and precise regulation of embryo development. These results suggest the bEECs regulates MMPs in the endometrium under progesterone-rich conditions and may thereby modulate bovine endometrial cell functions during gestation.