원문정보
초록
영어
Osteoblast differentiation can be suppressed by endoplasmic reticulum (ER) stress and ATF3 can be stimulated by inducing cytokines, hormones, DNA damage and ER stress. Up to date, the effect of ATF3 on osteoblast differentiation is not clear. To identify the correlation between ATF3 and ER stress on osteoblast differentiation, tumicamycin (TM) was treated to induce ER stress. TM up-regulated ATF3 expression in the pre-ostoblast cell line, MC3T3-E1 cells. Over-expressed ATF3 inhibited BMP2 and ALP, osteogenic markers. Also, suppression of ALP expression by TM was recovered by reducing ATF3 by shRNA. Here, our study shows that The stress-responsive transcription factor ATF3 is a negative regulator of osteoblast differentiation in MC3T3-E1 cells and over-expression of ATF3 inhibits BMP2-stimulated osteoblast differentiation via regulating alkaline phosphatase (ALP) expression and activation