earticle

영어

The main objective of this study is to investigated the expressions of apoptosis associated gene that are known to programmed cell death caused by mRNA expressions from two MMPs involved in degrading collagen and the basal membrane in cultured luteal cells on the treatment media. Our results found that the activity of MMP-2 gelatinase was higher in the last stage(CL2 and CL1) of luteal phase, and MMP-9 expression was higher in early stage of luteal phase. Especially, in the 72hr cultured cell of TIMP-3 expression patterns was highly, but that it's were lowly expression than TIMP-2 expression patterns. For the RealTime-PCR results, found that the MMPs and TIMPs pattern at the cultured luteal cells showed the same results in immune- detected of luteal cell. The expression of LH-receptor protein in the CL stages was more readily induced than that of the CH stages. For the FSH-receptor expression levels in CL2 and CL1 stages was most highly than to another stage. And MMP-2 and active MMP-2 enzymes was very higher activation from CL2 to CL1. However, activation levels of MMP-9 enzyme were decreased from CH2 to CL1. Also, the mTOR, MMP-9 and TIMP-2 expression were decreased from 24hr to 96hr cultured luteal cells, but for the MMP-2 and TIMP-3 expression were increased. The MMPs activation patterns from the zymography’s showed the same result as other experiment. These results indicated that active MMPs differential expressed tend to induce expression of genes associated with programmed cell death from degradation luteal cell.