earticle

영어

Transcription factors play a very important role in the development of embryo. It is different for each growth stage that position and expression level of the transcription factor. Also, it is different pattern in species of mammals. So we thought that if we could well know the expression of the transcription factor in the stage of embryo development, it would be more benefit in embryo culture system. Because the stages of early embryo development are basically from oocyte to blastocyst, we checked transcription factors from oocyte to blastocyst how are expressed in pig. We focused on pluripotency genes, which play an important role in early embryo development, among the transcription factors. So we were conducted to immuno-staining using antibodies of CDX2, OCT4, SOX2, NANOG, and E-CADHERIN. Unfortunately we used mouse`s antibody because pig`s antibody against transcription factors did not exist. We identified five transcription factors (CDX2, OCT4, E-CADHERIN, NANOG, and SOX2) in early development of oocyte embryo development in pig. Our results showed not only similar patterns but also different patterns between pig and mouse. Therefore, we have to investigate that different patterns of transcription factors play a role in pigs, and why come out.