earticle

영어

GRP78 is typically expressed in mammalian cells and plays a prominent role in the maintenance of cell homeostasis and development. Additionally, chicken serum-mediated proliferation has regulated the chGRP78 to control prevented apoptosis in chicken cells, thus allowing the required chGRP78-mediated anti-apoptosis. However, the precise molecular mechanisms underlying the chGRP78-mediated apoptosis process remained to be defined. Previously, we reported the effects of chGRP78 on chicken embryo fibroblast (CEF) and DF-1 cell proliferation. Here we identify the AKT1 as a key target of chGRP78 in apoptosis mechanism. Using immunoprecipitation and peptide sequencing, chGRP78 binding sites were detected in AKT1-mediated protein. chGRP78 causes activation of AKT1, and chGRP78 knockdown cells show a decreased level of AKT1. Importantly, activation of AKT1 depletion reciprocally inhibits the apoptosis and promotes proliferation in chicken cells. Taken together, AKT1-mediated signaling pathway plays a critical role in chGRP78-stimulated fibroblast survival and anti-apoptosis, which, suggests an important implications of our findings for the maintenance of chicken fibroblast cells through the inhibition of apoptosis.