earticle

영어

Regulation of bovine interferon-tau (bIFNT) production, a conceptus secretory protein implicated in the process of maternal recognition of pregnancy, However, its expression in vivo and its transcriptional regulation are not yet well characterized. Among more than bIFNT gene sequences characterized, approximately 90% of bIFNT transcripts expressed in utero is derived from bIFNT1 gene and amounts of transcripts from other bIFNT genes such as new type I bIFNTcl. It was hypothesized that the variation in expression levels exhibited by the bIFNT1 and bIFNTc1 genes was due to identical in the proximal promoter regions of these bIFNT genes. In the present inverstigation, the 5’-upstream (positions ‒1,000 to +51) region of bovine IFNT1 and IFNTc1 genes were analysed using bovine ear-derived fibroblast (EF) cell line, which would provide a more suitable system for studies of the bovine trophoblast transcription factor ETS2. Luciferase assay, the 5’-upstream region (positiond ‒1,000 to +51) of bIFNT1 and bIFNTc1 was then truncated at positions ‒637, ‒389, ‒262, ‒222, or ‒157, or point-mutated at potential ETS-binding site. As compared to the wild-type constructs, those with deletion and mutation constructs were reduced bIFNT transcription similarly. Also, these findings were confirmed by mutated examining idented ETS2 binding site on the bIFNT1 and bIFNTc1 genes 5’-upstream region, ETS2 transcription factor expression constructs revealed the importance of a ETS2 binding site at (between -79 to -70) was idented in the bIFNT1 and bIFNTc1 genes. These results demonstrate these the short promoter region including the ETS2 binding site, gene display identical by transcription factor ETS2, tissue-specific expression and developmental regulation during pregnancy, in determining bovine IFNT genes transcription by the trophectoderm.