earticle

영어

Mastitis in dairy animals is a costliest disease worldwide and caused by more than 150 micro-organisms. Mastitis is defined as inflammation of mammary gland which is characterized by pathological changes in mammary gland and physical and chemical changes in milk. Cow heifers are the future milk producers of every dairy herd. Heifer mastitis is a disease that potentially threatens production and udder health in the first and subsequent lactation leading to economic losses for dairy farms. Unfortunately, most producers regard young heifers as uninfected, and the presence of mastitis is not observed until calving or until first signs of clinical mastitis in early lactation. Therefore, present study was focused to understand the distribution pattern of mastitis etiological agents in cow heifer’s with their molecular characterization. We found Coagulase Negative Staphylococci (CNS) as chief etiological agents of heifers mastitis followed by Staphylococcus aureus, Streptococcus spp., E. coli etc. Moelcular characterization of most prevalent bacteria was done using bacterial specific genes such as nuc gene, E. coli gene, S agl gene etc. Effective treatment of mastitis is major challenge to field veterinarians, researchers and farmers, use of traditional antibiotics have only upto 60% care rate due to various factors. Hence, we also focused to find out the alternative ways to fighting against low cure rate by developing nano-particles based approaches to improve the treatment of mastitis. We used chitosan nano-particles as vehicles for the Ciprofloxacin. Chitosan-Ciprofloxacin nano-particles were synthesized and characterized for in vitro use as antibacterial agent against mastitis origin bacteria. Present study found that Chitosan-Ciprofloxacin nano-particles had good antibacterial activity with the slow release properties.