earticle

영어

Vitrification is a common technique of cryopreservation in animal genetic resources of oocytes or embryos and replacing slow freezing methods. This study examined the factors of preparing open pulled straw (OPU) that could be used for vitrification with digital heating gun (DHG). Thermal performance of heat transfer of DHG is more stable and adjustable than traditional way of heating method like hot-plate or alcohol lamp. Thermal setting value of DHG was adjusted to 250, 350, 400 and 450℃ and production rate of wanted OPS was examined. The thickness of wall and outer diameter of OPU were measured by microscopy. OSP production efficiency of 0.5 mL straw was observed at 0, 66.1, 90.5 and 85.7% for 1~2 sec and 9.5, 33.3, 47.6 and 23.8% for 2~3 sec of heating time. OPS with inner diameter in the range of 200~650 μm was produced with 0.5 mL straw, and OPS with inner diameter in the range of 150~300 μm was obtained with 0.25 mL straw. OPS at the desired length and size could be obtained easily when straw was heated for 1.6~2.3 seconds at 350~400℃. Production rates of 0.25 mL straw were higher than those of 0.5 mL straw, so it was needed more attention to control time and power that were required for optimal heat transfer with 0.5 mL straw. Based on the data obtained in this study, hand-made OPSs could be produced for vitrification devices to freeze COCs, oocytes and embryos of with various size of outer diameter.