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Poster Presentation : Cloning

C-Phycocyanin Supplementation during In Vitro Maturation Enhances Pre-Implantation Developmental Competence of Parthenogenetic and Cloned Embryos in Pigs

초록

영어

C-Phycocyanin (C-PC), a protein from green microalgae, has been suggested to possess various biological activities, including antioxidant and free radical scavenging properties. The aim of the current study was to explore the effects of C-PC on the maturation of porcine oocytes and subsequent developmental competence after parthenogenetic activation and somatic cell nuclear transfer (SCNT) as well as the underlying mechanisms. There was no significant improvement in nuclear maturation rates between the control and C-PC supplementation groups (1, 3, 5, 10 μg/mL). However, supplementation of 5 μg/mL C-PC in the maturation medium significantly increased blastocyst formation and hatching rates after parthenogenetic activation (59.6±3.6% and 33.0±2.6% vs. 49.8±3.5% and 27.4±2.4%, respectively). In addition, the presence of CPC during the maturation period significantly improved blastocyst formation rates and total cell numbers after SCNT (24.8±1.9% and 42.2±3.3 vs. 21.6±2.2% and 39.5±3.4, respectively) compared to the control group. Furthermore, cellular proliferation and the expression of pluripotency-related genes (SOX2 and NANOG) were increased in cloned blastocysts derived from the C-PC supplemented group. Importantly, C-PC supplementation during maturation not only improved cumulus expansion and increased the expression of cumulus expansion-related genes (HAS2, PTX3, and PTGS2), but also enhanced antioxidant capacity, improved mitochondria function, and decreased cathepsin B activity in porcine oocytes. These results demonstrate that C-PC may be useful for improving porcine oocyte quality and subsequent developmental competence in embryos.

저자정보

  • Shuang Liang Department of Laboratory Animal Center, College of Animal Sciences, Jilin University, Changchun, 130062, China. Department of Animal Science, Chungbuk National University, Cheongju 361-763, Republic of Korea
  • Jing Guo Department of Animal Science, Chungbuk National University, Cheongju 361-763, Republic of Korea
  • Yong Xun Jin Department of Laboratory Animal Center, College of Animal Sciences, Jilin University, Changchun, 130062, China
  • Bao Yuan Department of Laboratory Animal Center, College of Animal Sciences, Jilin University, Changchun, 130062, China
  • Jia-Bao Zhang Department of Laboratory Animal Center, College of Animal Sciences, Jilin University, Changchun, 130062, China
  • Nam-Hyung Kim Department of Animal Science, Chungbuk National University, Cheongju 361-763, Republic of Korea

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