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영어

Cordyceps militaris is distributed world wide and cultivated as a medicinal mushroom having many valuable biological and pharmaceutical activities. In the breeding of new C. militaris mushroom, single ascospores were isolated and tested their characteristics such as mycelial growth, mycelial density, fruiting body formation ability, the production of perithecia. As a heterothallic and bipolar species, C. militaris has two mating type loci consisting of opposite mating type idiomorph MAT1-1 and MAT1-2. Two opposite mating types were assayed using two sets of primers specific for C. militaris, which were amplified a 191-bp fragment for MAT1-1 and 233-bp fragment for MAT1-1. In the PCR tests, 8 MAT1-1 and 8 MAT1-2 were detected and most of 64 mating combinations were resulted in F1 hybrid mycelia and some of F1 hybrids produced high quality club-shaped, bright orange-red fruiting bodies in artificial media.