원문정보
초록
영어
The fatty acid composition of shiitake mushrooms cultivated at the mushroom farms under the identical condition was analyzed using a GC-FID. The lipids in mushrooms were extracted with a solvent mixture of methanol and chloroform. The lipids were saponified and methylesterified to make fatty acid methylesters (FAME). The FAME was isolated, identified and quantified using a GC-FID with a highly polar cyano-siloxane type capillary column. Linoleic acid was the most abundant fatty acid, representing about 74-81% of total fatty acids. Palmitic acid was the second most abundant fatty acid found in shitake mushrooms. The composition of fatty acid metabolite profiles were not greatly different with harvesting cycles. However, the contents of fatty acids in mushroom was greatly dependent on the harvesting cylcles. The fatty acid of contents in mushrooms obtained at the 1st harvesting cycle was much higher than those in mushrooms obtain at the 3rd harvesting cycle.