earticle

영어

This study was carried out to investigate the production of health functional food components through the production of GABA after culturing mycelia of Lentinula edodes in a medium containing four kinds of amino acids. In order to confirm the content of GABA in the medium, the amount of GABA produced by adding 0.1 M of Glutamic acid, Alanin, Glycine, and Lysine to PDA medium and PDB medium was confirmed. The amount of mycelia in the PDB medium was 4.85 ± 23 g/L in the amino acid-free medium, 5.12 ± 32 g/L in the glutamic acid medium, 4.63 ± 12 g/L in the Alanin medium, 4.87 ± 27 in the Glycine medium, And 4.78 ± 43 g/L in addition medium. The amount of amino acid added did not interfere with the normal growth because the amount of excess amount of amino acid was not significantly different from that of control. The content of GABA in the medium was 10357.32 ± 22 g/L in the amino acid-free medium, 30293.83 ± 45 g/L in the glutamic acid-supplemented medium, 11697.79 ± 34 g / In the medium was 10619.79 ± 37 and 3960.12 ± 23 g/L in the medium supplemented with Lysine. The content of GABA in the medium was 10357.32 ± 22 g/L in the control, 30293.83 ± 45 g/L in the glutamic acid supplemented medium, 11697.79 ± 34 g/L in the alanin supplemented medium, 10619.79 in the glycine supplemented medium ± 37 and 3960.12 ± 23 g/L in Lysine supplemented medium. These results suggest that the excess of glutaminic acid was the highest level of GABA in the mushroom culture medium. On the other hand, it was confirmed that the addition of Alanin and Glycine excess did not affect GABA production compared to Control. These results suggest that continuous GABA production could not be achieved by using ion exchange resin after disruption of GABA production by biological methods, but continuous GABA production using mycelium of Lentinula edodes was possible in this study