earticle

영어

The genotyping of segregation population, 110 monokaryotic progenies derived from F1 Pleurotus ostreatus hybrid between ‘Heuktari’ and ‘Miso’ (HMS012 × JHH021), was performed by PCR amplification with SSR primer sets. ‘Heuktari’ and ‘Miso’ showed distinct traits in fruiting body including cap color, yield and length. Those monokaryotic progenies were selected by median values in their fruiting body which made from selfing of 50 monokaryons of ‘Heuktari’ and ‘Miso’. A total of 546 SSR primers which were designed based on the genome sequences of two P. ostreatus monokaryons, JHH021 (from Heuktari) and HMS012(from Miso) were screened on HMS012 and JHH021. Of the 546 primers tested, more than 300 primers showed polymorphism on the parental strains. The hybrids made by crossing 110 monokarotic progeny from HMS012 x JHH021 strain with a monokarotic progeny from KNR2247 to access the quantitative trait. This study will be useful reference for QTL analysis of P. ostreatus.