earticle

영어

Alginate has been widely used in hydrogel- based tissue engineering area as an excellent carbohydrate biomaterial due to its good biocompatibility and easy gelation by cation induction such as calcium ions. 3D bioprinting has been regarded as a powerful tool for mimicking natural structure, because it can fabricate on-demanded hierarchical artificial tissues. Due to several advantages of alginate, it has been the most frequently used biomaterial in inkjet-based bioprinting area. However, this ionically crosslinked alginate IS noticeably impoverished under a physiological, aqueous conditions due to easy loss of calcium ions. Thus, the low structural integrity and poor mechanical stability limited long-term cell culture on alginate-based bioprinted structures. While barium or copper IOns can increase its stability, these also increase cytotoxic effects. In this work, we proposed a new visible light- activated crosslinkable alginate-based bioink, showing fast and non -cytotoxic gelation. We constructed 3D cell-laden structures using this novel alginate material and various cell types. The light/ionic double crosslinked alginate bioink showed excellent biocompatibility, increased mechanical properties, and improved dimensional stability. Further development of this novel alginate- based 3D inkjet bioink may facilitate preparation of transplantable human tissues.