원문정보
초록
영어
A functional glycan chip combined with on-chip enzymatic glycosylation was developed to prepare complex glycan sources and to apply glycan -involved applications simultaneously. Here, GM3 trisaccharide, GM2 tetrasaccharide, and GM1 pentasaccharide were successfully direct biosynthesized on lactose-immobilized surface through consecutive three glycosyltransferase reactions. Biosynthesized GM1-related complex glycans were demonstrated to provide the information on substrate specificity of whole cholera toxin. Thus, the proposed on-chip glycan biosynthesis system can provide a new direction toward obtaining complex glycan sources and complex glycan -involved applications such as glycan -protein interaction analysis and glycan biomarker-employed diagnosis.