earticle

영어

Understanding the carbohydrate-protein interactions provides useful information on cullular processes in living organisms and is also helpful for the understanding the secretion pathway with respect to stress. Ribosomal proteins are usually known as structural proteins for the synthesis of proteins when cells are under a normal condition. However, some ribosomal proteins have extra-ribosomal functions in case of stress conditions. For example, ribosomal protein S3, a multi-functional protein, is also involved in DNA repair, apoptosis, cell cycle control etc. We have discovered that this protein forms a dimer and is secreted into medium after N -glycosylation only from various cancer cell lines as well as cancer patients but not in normal cells or people who do not have cancers. We also have found that other ribosomal proteins such as S2, S20 and Rackl from human and yeast cells are also secreted under various stress conditions. The secretion pathway appears to be a standard ER -Golgi dependent pathway. We are currently investigating the glycosylation mechanism of these ribosomal proteins which could be used as useful biomarkers.