earticle

영어

In the production of therapeutic glycoprotein using recombinant CHO(rCHO) cells, sialylation is the critical factor in terms of biological functions, Previously, it was suggested that the sialic acid of glycoproteins produced from rCHO cells can potentially be impaired by sialidase released upon cell lysis during CHO cell culture, Bcl-xL overexpression in CHO cell culture not only increased Fc-fusion glycoprotein production, but also reduced the impairment of sialylation of Fc-fusion glycoprotein by maintaining high viability during the culture, Although the stressful conditions-induced cell membrane disruption can mainly lead to impairment of glycoprotein in CHO cell culture, rCHO cells maintained with viability more than 95% under some stressful condition can also produce impaired glycoprotein not caused by the release of intracellular enzymes such as proteases, From the NanoString nCounter analysis, we successfully identified differentially expressed N-glycosylation -related genes and miRN As to improve key bioprocess -relevant characteristics in the generation of engineered rCHO cells, Taken together, a number of factors can affect the sialylation of Fc-fusion glycoprotein produced from rCHO cells,