earticle

영어

Mouse has been used as an animal model for scientific research owing to its physiological similarity to human. However, their glycosylation of glycoproteins showed many differences between mouse and human. Usually N-glycosylation is directly involved in various biological process and plays crucial role in human diseases due to their unusual biological sensitivity. Therefore, the site-specific characterization of N-glycosylation in model mouse is necessary in order to investigate progression of disease. This study provided comparison of the site-specific N-and O-glycosylation between human and mouse plasma using LC-MS/MS with GPA (GlycoProteome Analyzer) system1.Thesite-specificN-andO-glycosylationbetweenhumanandmousehasthreedifferencesinpla sma.First,thesialicacidofN-andO-glycopeptideswasalmostentirelyNeu5Gcinmouseplasma,whileinh umanplasmawasNeu5Ac.Second,O-acetylatedNeuGcofN-glycopeptidewasidentifiedinmouseplasm aonly.Third,antennalHexNAc-NeuGcofN-glycopeptidewasspecificallyidentifiedfromseveralglycop roteinsinmouseplasmaonly.Inconclusion,ourstudyhasprovidedanoverviewofthesite-specificN-and O-glycosylationofhumanandmouseinplasma.