earticle

영어

Raw potato starch-based feeds controlled body weight and insulin resistance in mice. To investigate the association of the phenotypic changes with an impact on the gut microbiota, various starches-based feeds formulated based on the nutritional composition of AIN93G but substituted corn starch to wheat, rice, or potato starch, were provided ad libitum 6 week-aged C57BL/6 male mice for 16 weeks. Profiles of microbiota in the mice’s feces were compared by analyzing16s rRNA gene amplicon sequencing and QIIME pipeline with Greengenes database. It found that gut microbiota was changed according to the kinds of starches. The ratio of Firmicutes to Bacteroidetes was decreased by feeding potato starch diet and taxonomy assignment showed that potato starch diet increased the abundances of Akkermansia, Rikenellaceae and Sutterella. The large intestine of mice fed potato starch-based diet was heavier than those of other starches-fed mice. According to the literatures, it reported that Akkermansia and Sutterella are associated with improvement of obesity and especially, Akkermansia known to be correlated with the abundance of Rikenellaceae have been demonstrated to induce an increase in the large intestine weight and an increase of insulin sensitivity. Therefore, it supposes that the effects of potato starch based-diet on body weight and related insulin resistance can be elucidated from the intestine microbial changes.