원문정보
초록
영어
We have developed GlycoProteomeAnalyzer (GPA) for high throughput identification and quantification of N- and O-glycoproteins. GPA is a program for automatic identification of the glycan compositions and glycopeptide sequences by using three different scoring systems such as M-score for glycopeptide selection from oxonium-ions, S-score for theoretical isotope pattern match of molecular ion, and Y-score for glycopeptide identification from MS/MS fragmentation.[1] We here present the IQ-GPA Cloud which is a web based GPA application for automatic identification and quantification of site-specific N- and O-glycoproteins. IQ-GPA Cloud is a publicly accessible web application that enables users to quickly provision on-demand infrastructures for high-performance gylcoproteome analysis using Microsoft Azure cloud platforms. Users have instant access to a range of a glycan database builder as well as Nand O-GPA modules. IQ-GPA Cloud has been designed to easily handle high-throughput glycoproteomic data with graphical user interfaces. Microsoft Azure Cloud provides more than thousands of CPU to increase throughput of data analysis. Therefore, users can use glycoproteome analysis easily through IQ-GPA Cloud without any high-powered computer. The IQ-GPA Cloud is demonstrated on the website, https://cloud.igpa.kr/