원문정보
초록
영어
Glycosyltransferase from Bacillus licheniformis DSM13 (YjiC) was expressed in Escherichia coli BL21 (DE3) for enzymatic modification of emodin, aloe-emodin, alizarin, antraflavic acid and 2-amino-3-hydroxyanthraquinone in vivo. The recombinant strain successfully modified the above mentioned substrates to their glycosylated products by using their indigenous UDP-glucose for glycoconjugation. Additionally, their major glycosylated products exhibited the highest stability at pH 8.0 and temperature up to 70 °C and 60 °C respectively. Furthermore, the biological activities of emodin and its major glucoside (P1) were compared and their anti-cancer activities were assayed in several cancer cell lines. The results demonstrate that YjiC has the capacity to catalyze the glycosylation of these aromatic compounds and that glycosylation of anthraquinones enhances their aqueous solubility while retaining their biological activities.