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영어

A cheap UDP-D-glucose producing system was developed by using sucrose as primary source. The system was coupled with the UDP generating glycosylation reaction of UDP-D-glucose dependent glycosyltransferase (GT) enzymes mediated reactions. As a result, the UDP produced as by-product of the GT-mediated reaction was recycled. YjiC, a UGT from Bacillus licheniformis DSM 13 was used as GT for transferring glucose from UDP-glucose to resveratrol while AtSUS1 from Arabidopsis thaliana was used to hydrolyze sucrose to UDP-glucose and fructose. The established UDP-recycling system is highly feasible for large scale production of different derivatives of resveratrol and other natural products glucosides using inexpensive starting materials. The effect of glucosylation of resveratrol on macrophage cell viability and immunomodulation properties have been studied in murine macrophage RAW 264.7 cells. During the study, nitric oxide (NO) and interleukin 6 (IL-6) expression and production after treatment with (E)-resveratrol, (E)-resveratrol 3-O-β-D-glucoside (R-3-G) and (E)-resveratrol 4ʹ -O-β-D-glucoside (R-4ʹ-G) in vitro at different concentrations. In cell viability assays of macrophages, two different resveratrol monoglucosides, R-3-G and R-4ʹ-G, exhibited significantly reduced cytotoxicity in comparison to (E)-resveratrol standard. The conjugation of glucose moiety on resveratrol was found to enhance the immunomodulating activity and viability of RAW 264.7 cells. Glycoside derivatives of resveratrol have shown many potentiality of medicinal and biological activities.