earticle

영어

Generally glycosylated natural compounds have the specific contribution to the pharmacological properties. Particularly, C-glycosylflavones are involved in UV protection, defense against pathogens and inhibition of caterpillar growth. C-glycosylflavones are biosynthesized from flavone via C-glycosylation of 2-hydroxyflavone or flavone. Two plant C-glucosyltransferases (CGTs) UGT708D1 from Glycine max and GtUF6CGT1 from Gentian triflora were used for glucosylation of chrysin. UDP-glucose pool was enhanced by introducing heterologous UDP-glucose biosynthestic genes i.e., glucokinase (glk), phosphoglucomutase (pgm2), and glucose 1-phosphate uridylyltransferase (galU), along with glucose facilitator diffusion protein (glf), in a multi-monocistronic vector pIBR. Moreover, the bioengineered E. coli strains enhanced the production by approximately 1.4–fold, thus producing 10 mg/L and 14 mg/L by UGT708D1 and GtUF6CGT1, respectively, without supplementation of additional UDP-glucose in the medium. HPLC analysis of fermentation broth extract showed 50 % (42 mg/L) conversion. Thus in this study, we successfully biosynthesized C-glycosylflavone in vivo and the product was confirmed by LC-MS and NMR.