원문정보
초록
영어
Kaempferol is a natural flavonol with numerous bioactivities. It reduces the risk of various cancers and possess skin-lightening effect. Due to these efficacies, its possible application in food, medicine and cosmetics industry are increasing. However, like most flavonoids it has limited application due to water solubility and stability. Glycosylation has been an important approach to diversify and extend novel biological functions in natural products and secondary metabolites. Hence, in this study, considering these demerits, we synthesized astragalin (kaempferol glucoside) from kaempferol using uridine 5′-diphosphate (UDP)-3-O-glycosyltransferase. To enhance the pool of UDP-glucose necessary for UGT78K1, UDP-glucose biosynthetic genes along with glucose facilitator diffusion protein (glf) and glucokinase (glk) were expressed in a multi-monocistronic vector pIBR to produce astragalin in significant titer.