원문정보
초록
영어
The purpose of this experiment is to investigate the glycosylation using enzymes. E. coli BL21 (DE3) was used as a host for protein overexpression. Phloroglucinol (PG) is used as an antiseptic and the treatment of gallstones, cramps and gastrointestinal disorders. According to HPLC results, the glycosylation of PG quickly generated in a few hours. Pyrogallol is a medicinal product that is used in psoriasis and antimicrobial agents. We progressed this reaction with ascorbic acid which is antioxidant for reaction stability of phloroglucinol and pyrogallol. 2,4-dihydroxybenzoic acid are belong to flavor modulators. The pharmacological action of 2,5-dihydroxybenzoic acid has a wide range of biological activities such as anti-inflammatory, antirheumatic and antioxidant properties. When salicylic acid is glycosylated, we anticipate both antivirus and antipyretic effects against influenza. In the case of p-aminosalicylic acid, when glycosylated, we anticipate alleviation of various side effects mentioned above as a result of enhanced water solubility of drug. The glycosylation of salicylic acid by amylosucrase was not detected, but we observed through HPLC that the level of glycosylation of p-aminosalicylic acid, 2,4-dihydroxybenzoic acid, and 2,5-dihydroxybenzoic acid, although slow in reaction rate, increased over a time period.