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영어

Phenolic compounds are used as preservatives for cosmetics and antioxidants as well as for various biological activities such as anti-inflammation, anti-cancer, antioxidant and immunomodulation, and have many physiologically active functions. Hydroquinone inhibits skin melanin formation and brightens dark skin. Resorcinol is used to treat rough skin or cured skin, to disinfect the skin to prevent infection, to treat pain and itching caused by minor wounds and other skin irritations, and to treat other skin conditions. Catechol is reported to be an inhibitor of the pro-inflammatory pathway and can be considered a candidate for treating neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease and other inflammatory diseases. Despite the importance of these phenolic compounds, the use of these compounds is not well soluble in water, they are easily oxidized by light in aqueous solutions, and are generally limited due to toxicity and side effects. Amylosucrase (AS) from Deinococcus geothermalis (DGAS) was selected in the GH-13 line to address this problem. The advantage of the amylosucrase enzyme is that it has excellent activity, such as ADP- or UDP-glucose, but is industrially useful because it uses relatively inexpensive glycosyl donors such as sucrose as compared to expensive glycosyl donors. DGAS produced dihydroxybenzene glucoside, which was confirmed by high performance liquid chromatography (HPLC). Each reaction efficiency is hydroquinone glucoside (90%), resorcinol glucoside (70%), and catechol glucoside (90%). The major glycoside products are identified based on mass spectrometry.