earticle

영어

Resveratrol glycosides have been produced using the flexible glycosyltransferase gene YjiC from Bacillus licheniformis DSM-13. The reaction mixture containing purified YjiC, nucleotide diphosphate (NDP) D- and L-sugars as sugar donor and Resveratrol as acceptor substrate was incubated at 37 ℃for 5 hours.The spectrometric analysis HPLC-PDA, mass and nuclearmagnetic resonance analysis of the reaction mixture showed the production of glucose, galactose, 2-deoxyglucose, viosamine, rhamnose, and fucosesugar-conjugated resveratrol glycosides. α-D-glucose as the sugar donor gave the higher(90%) bioconversion of resveratrol producing four different glucosides of resveratrol, resveratrol 3-O-β -D-glucoside, resveratrol 4′-O-β-D-glucoside, resveratrol 3,5-O-β-D-diglucoside and resveratrol 3,5,4′-O-β-D-triglucoside. In the case of other NDP-sugar donors, the conversion rates and the number of products formed were varied. While using TDP-2-deoxyglucose as sugar donor, Resveratrol 3-O-β-D-2-deoxyglucoside, resveratrol 3,5-O-β-D-di-2-deoxyglucoside were found to be produced, however ,monoglycosides resveratrol 4′O-β-D-galactoside, resveratrol 4′O- β-D-viosaminoside, resveratrol 3′O-β -L-rhamnoside, resveratrol 3′O- β-L-fucoside were produced from respective sugar donors. On the whole, ten diverse glycoside derivatives of medicinally important resveratrol were generated. This demonstrated the capacity of YjiC to produce structurally diverse resveratrol glycosides.