earticle

영어

Antibody has been extensively investigated and developed as new drugs for many diseases, In-depth biological and structural analyses are needed for developing antibody drugs compared to small molecule drugs. Therefore, wide investigation of antibody with liquid chromatography (LC) and mass spectrometry (MS) have gained attention for the needs due to its sensitivity. Profiling of N-linked glycans and glycosylation site analysis are one of the major parts among the antibody physico-chemical characterization. In present works, glycan profiling is performed through deglycosylation, labeling and purification with Waters RapiFluor-MS N-glycan kit, then UPLC-FLR analysis gives more profiling results compared to conventional 2-AB method. A total of forty-one N-glycan was identified and a total of sixteen N-glycan were relatively quantified within 1% RSD for commercial antibody drugs. Glycosylation sites are easily identified with glycopeptides analysis and PNGase F treatment. Both LC-MS based analytical methods improved understanding of the antibody drugs and will be applicable to non-commercial antibodies or other biological drugs including biosimilars.