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Background & objectives: Life style modification reduces the health risks of young people and improves their health status. Physical activity is known the effective factor to reduce health risks and health problems. This study performed to analyze health promotion behaviors related to physical activity among first-year students of university. Methods: To examine the association with physical activity and health promotion practices, health survey was conducted with 3,806 students who were first-year students in a university by self-reported questionnaire from February 26 to March 10, 2015. Multiple logistic regression analysis was performed to identify the difference of health promotion behaviors by physical activities. Results: 51.4% of the first-year student was regular exercisers who had practiced on regular walking exercise or regular strength exercise or regular aerobic exercise for a week. 87.8% of students exercised one or more times within the last one week. On the other hand, 12.2% of them did not exercise. In multiple logistic regression models, it remained significantly the difference of regular exercise by gender, subjective health status, sleeping time per one day, BMI, drinking behavior, and eating habit (p<0.05, p<0.01, p<0.001). Also it remained significantly the difference by physical activity type, such as walking, strengthen, and aerobic exercise (p<0.05, p<0.01, p<0.001). Conclusion: To help the achievement of academic goal, it should build physical activity policies and comprehensive health promotion programs to reduce health risk factors of university students. Comprehensive university health services and customized program for university students could help to make the best of their health. In future, to enhance physical activity practice, it needs to develop various tailed messages and smart healthcare service using health information technology (IT) on campus.