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Purpose: The purpose of this study was to examine the relationship between perceived job stress and work environmental factors related with socioeconomics factors and physical burden in the golf caddies. Methods: Job stress in golf caddies was estimated by using the Karasek's job content questionnaire (JCQ) and work environmental factors were estimated by self-reported questionnaire and interview. Results: The subjects were 316 woman caddies who had never been occupationally exposed to hazardous chemicals, was mean age 31.1 years. Mean work duration was 6.44 years and working hours per day on low- and high-demand season were 6.16 and 10.51, respectively. The golf caddies displayed higher scores of job stress due to multiple factors such as working hours and frequency of rounding per week, physical burden, confliction, income satisfaction and employment stability. We found that high strain group was positive correlation with age, career, working hour (including frequency of rounding) and job demand, however, showed negative correlation with job decision latitude and support of supervisor and coworker. Conclusion: In conclusion, we suggest that the golf caddies be regard divided into high strain group as appropriate. Thus, stress management and health promotion program for golf caddies should be developed.