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Purpose: The purpose of this study was to examine the effects of the one-time aerobic exercise on pulmonary function, oxygen saturation, and smoking in male college students in their 20’s. Methods: The experiment was performed on 11 healthy men who had no musculoskeletal or neurological diseases and who smoked. The subjects performed an aerobic exercise for 30 minutes, using a step box. Before and after the exercise, forced vital capacity (FVC), forced expiratory volume for one second (FEV1), and oxygen saturation were measured. Results: Between the two measurements, there were no significant differences in oxygen saturation (p>.05). However, the differences in FVC and FEV1 were statistically significant (p<.05). Conclusions: The results of this study demonstrate that a one-time aerobic exercise improves pulmonary function.