원문정보
초록
영어
An efficient and reproducible protocol for in vitro germination of seeds of Couroupita guianensis has been developed in the present study. Couroupita guianensis is a medicinally important tree and categorized as threatened plant species by the IUCN. The seeds were surface sterilized using 0.1% HgCl2 and inoculated on MS (Murashige and Skoog) and Woody Plants (Llyod and McCown) medium. The seeds with partially removed seed coat responded better (100%) on half strength MS medium as compared to the full (51%), one-fourth strength MS (79%) and the WP medium. Half strength MS medium conjunct with 2.0 mgㆍL-1 indole-3 butyric acid (IBA) was found best among the different types of auxins and cytokinins tested for seed germination, as all the seeds germinated on this medium combination. The seedlings were successfully hardened in the greenhouse and restored in the field with 83% survival rate. The protocol can be used as conservation measure for large scale multiplication of this threatened forest tree species.
목차
Introduction
Materials and Methods
Collection and surface sterilization of seeds and experimentaldesign
The culture media
Effects of media strength, light and darkness on in vitro germination of seeds
Hardening and restoration of in vitro seedlings
Results and Discussion
Effects of media and media strength on germinationof seeds of C. guianensis
Effect of growth regulators on seed germination
Influence of photoperiod on germination of seeds
Hardening of seedlings and reintroduction of plantletsin field
Conflict of interest
Conclusion
Conflict of interest
Acknowledgements
Author contribution
References