This case report handled the comparative sequence analyses of plastid DNA markers acquired rom botanical stains of a suspect’s clothes and a sickle which were the items of criminal evidence in agricultural property damage case. Three victims found out that their cherry tomatoes in green houses of the width of about 5,950 square meters were cut off. For the forensic botanical analysis, the police requested a suspect’s clothes, cotton gloves, two sickles, gauzes and cotton swabs collected by wiping a suspect's motorcycle and car. Cherry tomato plants from victims’ green houses as positive controls were received. The nucleotide sequences of plastid trnL-trnF IGS and psbA-trnH IGS regions from evidences were compared with positive controls and with DNA databases in GenBank of National Center for Biotechnology Information (NCBI). According to nucleotide sequence results, the lower part of suspect’s training wear got stained with tomato substances, the upper part with kiwifruit. The wooden handle of a sickle of a suspect showed the apricot nucleotide sequences. Gauzes, cotton swabs, two gloves, two pants and other sickle showed no results by plastid DNA markers. Plant stains from clothes and wooden materials or wood itself can be genetically analyzed for the elucidation of criminal cases.
2.2 DNA 분리 및 증폭
2.3 염기서열 분석 및 데이터 해석