초록
영어
Pleurotus eryngii is one of the most commercially important mushrooms cultivated in Korea. However, the shelf-life of the fruiting body is short, limiting its export. A new hybrid strain H17 of P. eryngii was developed to extend the shelf-life by mono-mono crossing between monokaryotic strains derived from DanBi and KNR2774. Although the cultivation period of H17 was slightly longer than that of the reference cultivar Kenneutari No.2, the quality did not change and remained normal after a period of 65.0 days at 4°C. This result was significantly different from that of the reference cultivar Kenneutari No.2. Analysis of the genetic characteristics of the new hybrid strain H17 revealed a different profile from that of the parental and reference cultivars when random amplification of polymorphic DNA (RAPD) primers was used. These results demonstrate that H17 is a new cultivar with improved storability after harvesting.
목차
INTRODUCTION
MATERIALS AND METHODS
Strain and growth conditions
Single sporing and crosses
Fruiting and cultivation methods
Hardness and storage on fruiting body
Antagonistic test and random amplification ofpolymorphic DNA (RAPD) analysis
Statistical analysis
RESULTS AND DISCUSSIONS
Strains produced from mono-mono crosses of DanBiand KNR2774
Fruiting body production and storage of the newdikaryon strains
Antagonistic test
RAPD analysis
Relation between shelf-life and hardness of fruitbody
ACKNOWLEDGEMENTS
REFERENCES