earticle

영어

Recently, there have been many efforts to develop new compounds having skin whitening effects from natural products. The aim of this study was to assess the availability of Asterubine extracted from Asterias amurensis as a skin whitener through the evaluation of the inhibitory effects of melanogenesis in B16F1 melanoma cells. B16F1 cells were treated with Asterubine extracted from Asterias amurensis (10, 100 and 1,000 μM/mL, respectively) and incubated for 72 hours. B16F1 cells were treated with Asterubine (10, 100 and 1,000 μM/mL, respectively) and α-melanocyte stimulating hormone (α-MSH), simultaneously, and incubated for 72 hours. Content of melanin synthesis and tyrosinase activity were determined. The expressions of tyrosinase was measured by Western blot analysis. Treatment with 100 and 1,000 μM/mL Asterubine significantly decreased tyrosinase activity by 11.4% and 27.7%, respectively, compared to Asterubine non-treated group (p<0.05). Treatment with 1,000 μM/mL Asterubine significantly decreased melanin synthesis by 26.5%, compared to α-MSH alone group (p<0.05). Asterubine significantly inhibited expressions of tyrosinase induced by α-MSH. In conclusion, these findings indicate that Asterubine extracted from Asterias amurensis, which inhibits melanin synthesis and tyrosinase expression, is an effective skin whitening agent.