원문정보
초록
영어
This study investigated the effect of type II collagen extract on SD rats with deteriorated immunity caused by methotrexate. The test samples were dosed once a day for 28 days by gastric gavage at dosage 250 mg/kg and 500 mg/kg after methotrexate treatment, and the changes on body weight, total blood leukocyte numbers, the percentages of B-cells, CD4+ T-cells and CD8+ T-cells in the blood and spleen were observed. The changes on body weight, the total blood leukocyte numbers, the to-tal lymphocyte numbers in the spleen, the ratio of CD4+ and CD8+ T-cells in the blood and spleen were increased significantly in type II collagen extract groups as compared with the control group. According to the above results, type II collagen extract has an effect of increasing immune responses on rats with deteriorated immunity caused by meth-otrexate.
목차
INTRODUCTION
MATERIALS AND METHODS
Animals and reagents
Classification of experimental groups
Immune degradation using MTX
Reagent administration
Measurement of body weight
Blood sampling
Preparation of splenocytes
Measurement of B cell ratio in the spleen
Measurement of CD4+ and CD8+ T cell ratio in spleen and blood
Cell culture
Cell viability measurement
Nitric oxide production measurement
Statistical analysis
RESULTS
Body weight change measurement
Changes in total leukocyte counts
Measurement of CD4+ cell ratio in blood
Measurement of CD8 + cell ratio in blood
Measurement of B cell ratio in the spleen
Measurement of CD4+ cell ratio in the spleen
Measurement of CD8+ cell ratio in the spleen
Measurement of cell viability
Measurement of NO production
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES