원문정보
초록
영어
Epilepsy is characterized by recurrent seizures and loss of neurons with abnormal rhythmic firing in the brains. In the present study, we investi-gated the effect of treadmill exercise on memory function in relation with cell proliferation and apoptosis in the hippocampus using pilocar-pine-induced seizure rats. Epilepsy was initiated by intraperitoneal in-jection of pilocarpine hydrochloride. The rats in the exercise group were forced to run on a motorized treadmill for 30 min once a day for 2 weeks. In the present results, treadmill exercise alleviated short-term and spatial learning memory impairments in the epileptic rats. Treadmill exercise suppressed neuronal degeneration and enhanced neuronal maturation in the epileptic rats. Treadmill exercise suppressed cell pro-liferation and apoptosis in the epileptic rats. Treadmill exercise alleviat-ed pilocarpine-induced memory impairments and suppressed neuronal loss in the hippocampus through down-regulation of apoptosis. These findings offer a possibility that treadmill exercise may provide a preven-tive or therapeutic value to the epilepsy-induced learning and memory impairments.
목차
INTRODUCTION
MATERIALS AND METHODS
Experimental animals
Pilocarpine-induced epilepsy
Treadmill exercise protocol
Step-down avoidance task
Radial-arm maze task
Tissue preparation
BrdU Immunohistochemistry
Immunohistochemistry for NeuN, caspase-3, DCX
TUNEL staining
Fluoro-Jade B staining
Western blot analysis
Statistical analysis
RESULTS
Latency of the step-down avoidance task
Correct number and error number of the radial-arm maze task
Fluoro-Jade B-positive and NeuN-positive cells in the hippocampal CA1 region
BrdU-positive and DCX-positive cells in the hippocampal dentate gyrus
Caspase-3-positive and TUNEL-positive cells in the hippocampal CA1 region
Bax and Bcl-2 expressions in the hippocampus
DISCUSSION
CONFLICT OF INTEREST
REFERENCES