원문정보
초록
영어
The red algae Gracilaria vermiculophylla is widely spread around seaside areas across the globe, and has been used as a food resource in Southeast Asian countries. Previous studies have shown that Gracilaria red algae extracts have beneficial antihypercholesterolemic, antioxidant, anti-inflammatory, and antimicrobial effects. In this study, we investigated the antioxidant effects of Gracilaria vermiculophylla extracts (GV-Ex) on human bone marrow mesenchymal stem cells (hBM-MSCs). The acetone and DMSO/ethanol solvents of the tested GV contain higher total flavonoid and polyphenolic contents that can strongly scavenge reactive oxygen species (ROS). Pre-treatment with GV-Ex protected hBM-MSCs against oxidative stress induced by hydrogen peroxide treatment. The protective effects of GV-Ex treatment were confirmed by MTT assay. The elevated levels of ROS in hBM-MSCs caused by hydrogen peroxide induced oxidative stress were significantly decreased by GV extract treatment. The levels of the antioxidant proteins superoxide dismutase 1 (SOD1), superoxide dismutase 2 (SOD2), and catalase (CAT) were also restored or protected by GV-Ex treatment, suggesting that GV extracts moderate excess ROS levels and prevent cells from oxidative damage.
목차
1. Introduction
2. Materials and Methods
2.1. Characteristics of Primary hBM-MSCs and Cell Culture
2.2. Preparation of Gracilaria Vermiculophylla Extract
2.3. Determination of Total Polyphenolics Compounds and Flavonoids Contents
2.4. Antioxidant Assays
2.5. MTT Assay
2.6. Detection of Intracellular ROS
2.7. Immunoblot Analysis
2.8. Statistical Analysis
3. Results and Discussion
3.1. Total Polyphenolic Content (TPC) and Total Flavonoids Content (TFC)
3.2. Antioxidative Activity
3.3. Protection by GV Extracts in H2O2-treated MSCs
3.4. Intracellular ROS Levels were Moderated in GV-treated MSCs
4. Conclusions
Acknowledgements
References