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Objectives: The purpose of this study was to explore and describe the influencing factors in preventive behavior of tuberculosis among elementary school teachers. Methods: Participants were 151 teachers working at 3 elementary schools in Kimhae City, 3 in Changwon City and 2 in Miryang City. Data were collected with structured questionnaires from May. 8. to Jun. 1. in 2018. Data were analyzed by SPSS/WIN 21.0 program for descriptive statistics, t-test, ANOVA, Pearson's correlation coefficient and stepwise multiple regression. Results: The preventive behavior of tuberculosis among elementary school teachers were significantly positive correlated with knowledge(r=.275, p=.001), attitude(r=.493, p<.001) and self-efficacy(r=.280, p=.001). Moreover, The significant factors of the preventive behavior of tuberculosis were attitude(β=.341, p<.001), gender(β=.407, p<.001), smoking status(β=.210, p=.004), self-efficacy(β=.187, p=.005) and knowledge(β=.145, p=.026). Conclusions: In this study, in order to promote the preventive behavior of tuberculosis among elementary school teachers, it is necessary to find ways to increase knowledge, attitude, and self-efficacy of tuberculosis, which are significant influencing factors. In addition it would be possible to develop more effective and efficient nursing intervention program if the individual characteristics of the subjects such as gender and smoking status are considered.