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Purpose: The purpose of this study was to investigate the effect of muscle activity and balance when the women’s university students trained through bio-feedback. Methods: Fourteen healthy 20~30-year-old women participated in the study. Subjects were divided into two groups. The control group (n =7) did not undergo exercise and/or stimulation. The bio-feedback training group (n =7) underwent stimulation while looking at the screen using the bio-feedback balance system (3 times/day for 4 weeks). All tests were completed before and after the experiment. Muscle activity was measured using surface electromyography (Bagnoli EMG system, Delsys Inc. USA). Balance ability was measured by the GOOD BALANCE system (Metitur Ltd, Finland). Results: There were significant improvements in the muscle activity and balance ability tests for those in the bio-feedback training group (p <.05), while the control group showed no significant changes (p >.05). Conclusion: The above results revealed that bio-feedback training can improve the muscle activity and balance ability of functional ankle instability in women’s university students.