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Original Article

Treadmill exercise with bone marrow stromal cells transplantation facilitates neuroprotective effect through BDNF-ERK1/2 pathway in spinal cord injury rats

원문정보

You-Mi Kim, Jun-Jang Jin, Sam-Jun Lee, Tae-Beom Seo, Eun-Sang Ji

한국운동재활학회 JER Vol.14 No.3 2018.06 pp.335-340
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초록

영어

Transplantation of bone marrow stromal cells (BMSCs) has been known as one of the effective therapeutic methods for functional recov-ery of spinal cord injury (SCI). Treadmill exercise also facilitates the functional recovery of SCI. Previously, we reported that combination of BMSCs transplantation with treadmill exercise potentiated the locomo-tor function in SCI rats. In the present study, we investigated whether recovery effect of BMSCs transplantation or treadmill exercise appears through the brain-derived neurotrophic factor (BDNF)-extracellular sig-nal–regulated kinases 1/2 (ERK1/2) pathway. The spinal cord contusion injury was performed at the T9-T10 level using the impactor. Cultured BMSCs were transplanted directly into the lesion 1 week after SCI. Treadmill exercise was performed 6 days per a week for 6 weeks. Western blot for Bax, Bcl-2, BDNF, tyrosine kinase B (TrkB), and phos-phorylated ERK1/2 (p-ERK1/2), phosphorylated JNK was performed. In the present results, combination of BMSCs transplantation with tread-mill exercise potently decreased Bax expression, potently increased Bcl-2 expression, and potently enhanced BDNF and TrkB expressions in the injured spinal cord. Combination of BMSCs transplantation with treadmill exercise further facilitated p-ERK1/2 and p-c-Jun expression levels. The present findings demonstrated the synergistic effect of treadmill exercise on neuroregenerative effect of BMSCs transplanta-tion appeared through the activation of BDNF-ERK1/2 pathway in SCI.

목차

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Animals and treatments
  Spinal cord contusion injury
  BMSCs culture and transplantation
  Treadmill exercise protocol
  Western blot analysis
  Data analysis
 RESULTS
  Bax and Bcl-2 expressions
  BDNF and TrkB expressions
  p-ERK1/2, p-JNK, and p-c-Jun expressions
 DISCUSSION
 CONFLICT OF INTEREST
 ACKNOWLEDGMENTS
 REFERENCES

저자정보

  • You-Mi Kim Sports Science Research Institution, Korea National Sport University, Seoul, Korea
  • Jun-Jang Jin Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea
  • Sam-Jun Lee Department of Physical Education, College of Health, Welfare, and Education, Tong Myong University, Busan, Korea
  • Tae-Beom Seo Department of Kinesiology, College of Natural Science, Jeju National University, Jeju, Korea
  • Eun-Sang Ji Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea

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