원문정보
초록
영어
Depression is a major cause of disability and one of the most common public health problems. In the present study, antidepressive effect of treadmill exercise on chronic mild stress (CMS)-induced depression in rats was investigated. For this, sucrose intake test, immunohistochem-istry for 5-bromo-2’-deoxyuridine, terminal deoxynucleotidyl transfer-ase-mediated dUTP nick end-labeling staining, and Western blot analy-sis for brain-derived neurotrophic factor, cyclic adenosine monophos-phate response element binding protein, and endothelial nitric oxide synthase were conducted. Following adaptation to the animal vivarium and two baseline fluid intake tests, the animals were divided into four groups: the control group, the CMS-induced depression group, the CMS-induced depression and exercise group, and the CMS-induced depression and fluoxetine-treated group. The animals in the CMS groups were exposed to the CMS conditions for 8 weeks and those in the control group were exposed to the control conditions for 8 weeks. After 4 weeks of CMS, the rats in the CMS-induced depression and ex-ercise group were made to run on a motorized treadmill for 30 min once a day for 4 weeks. In the present results, treadmill exercise alleviated CMS-induced depressive symptoms. Treadmill exercise restored su-crose consumption, increased cell proliferation, and decreased apop-totic cell death. The present results suggest the possibility that exercise may improve symptoms of depression.
목차
INTRODUCTION
MATERIALS AND METHODS
Animals and treatments
Sucrose intake test
Chronic mild stress
Treadmill exercise protocol
Fluoxetine treatment
Tissue preparation
BrdU immunohistochemistry
TUNEL staining
Western blot
Data analysis
RESULTS
Sucrose intake test
Numbers of BrdU-positive cells
Numbers of TUNEL-positive cells
BDNF expression
CREB expression
eNOS expression
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES