earticle

영어

Colchicine is a useful chemical used to induce polyploidy in plants. Diploids of C. unshiu 'Sangdojoseang' and C. hybrid 'Sagakashi No. 34' were treated with colchicine to develop tetraploids for breeding of seedless citrus varieties and their several characters were investigated. Buds on scions were treated with 0.1% colchicine solution twice for 15 minutes each time and grafted onto trifoliate orange rootstocks. As the result of polyploidy analysis of new leaves by flow cytometry, we got 1 tetraploid of 'Sangdojoseang' and 4 tetraploids of 'Sagakashi No. 34'. Tetraploids showed unvigorous growth compared with diploids. Leaves of tetraploids were thicker and blunter compared with diploids. Stoma density was lower and stoma size was larger than that of diploids. Tetraploids showed shorter length and larger width in flower bud, petal, pistil and ovary than those of diploids.