원문정보
초록
영어
The human gut contains an enormous number of microorganisms known as microbiome, which serve to modulate immune functions and metabolic pathways in the body. Studies have shown that there is a strong association between balanced diet and microorganism present in the gut. This study sought to determine the effect of childhood malnutrition on the gut microbiome in adulthood via mice models. DNA samples of gut bacteria were extracted from the feces of the nutrient-restricted mice and control group and analyzed by 16S rRNA gene sequence analysis. Cognitive functions and emotional behaviors were also evaluated in the mice by conducting modified Morris water maze and light-dark preference tests. The results show that the pathogenic bacteria, specifically Bacillus cereus, present in the experimental group was greatly increased compared to the control group. Furthermore, the nutrient-restricted mice showed a slow adaptation and emotional instability in the modified Morris water maze test and preferred light-exposed area in light-dark preference tests. Though further studies are necessary to prove causality, this study suggests that nutritional status during childhood might play a critical role in the composition of gut microbiome and influence cognitive functions and emotional behaviors in adulthood.
목차
1. INTRODUCTION
2. MATERIALS AND METHODS
2.1. Preparation of Animal models
2.2. Cultivation of the gut bacteria
2.3. Classification and Isolation of the colon bacteria
2.4. Bacterial DNA sequencing and Blast analysis
2.5. Evaluation on cognition function and emotional behaviors
2.6. Blood serum test
3. RESULTS
3.1. The effect of nutrient restriction on the body weight of mice models
3.2. The effect of nutrient restriction on the lactic acid bacteria in the gut
3.3. The effect of nutrient restriction in the Gut microbiome ofthe mice models
3.4. Psychological behavioral Changes in mice models
3.5. Blood Test Result obtained after the recovery period from nutrient restriction
4. DISCUSSION
5. CONCLUSION
6. REFERENCE