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Fruit waste could be used for producing ethanol in an efficient way by means of mashing specialized enzymes. In this paper fruit waste of oranges, apples and tangerines was processed simultaneously by saccharification and fermentation in a laboratory-scale. The enzymes used for this study are commonly-found C-Tec2 and H-Tec and Wild-type Saccharomyces Cerevisiae was the yeast used for ethanol fermentation. The experimental analysis was conducted using the 1260 Infinity Quaternary LC System® of the Agilent Technologies Co. The experinment results showed the glucose and ethanol production from oranges, apples, and tangerines it could be concluded that most of fruits showed decreasing trends of the glucose production and increasing trends of the ethanol production in 2 hours. After twenty four of incubation the glucose produced from fruits was mostly converted to ethanol. The study results suggest that fruit waste could be utilized as economically- as well as environment-friendly substrate by simultaneous saccharification and fermentation.