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Ambrosia trifida L, a large herbal plant of the compositae, has a high photosynthesis rate that enables the plant to grow tall and big. The tall and big leaves cover sunlight going to other plants, thus suppressing the growth of other plants and eventually simplifying the variety of neighboring plants. Moreover, Ambrosia triggers pollinosis by spreading its pollens. In order to reduce the population of Ambrosia, it was used as livestock feed, but the results showed limitations in actual use due to the low effectiveness. To find an effective method to resolve this problem, a study investigating the glucose content of Ambrosia and the effects when supplied to plants was conducted. In the experiment, glucose content was verified, and when given to plants, the extract made with the inner stem produced the longest growth length and highest chlorophyll content with a concentration of 50%. The use of Ambrosia in powder form also caused beneficial effects for the plant. When drying the residue formed after making an extract, the Ambrosia turned into paper form. The use of this Ambrosia paper also increased the chlorophyll content. Therefore, through the use of the inner stem of Ambrosia as a fertilizer, it can reduce the soil acidification caused by chemical fertilizers, resolve water contamination problems, and elevate the quality of crops.